Vasilieva Lab
RNA Regulation Laboratory
Selected publications:
Slade, L., Kus, K., Filipovic, M., Sarkies, P. and Vasiljeva, L. (2026) The Intergator complex couples Non-coding RNA biogenesis, mitochondrial function and innate immunity to control life and health span. BioRxiv, DOI: https://doi.org/10.64898/2026.04.18.719358
Ho, A., Southers, A., Kus, K., Schoemaker, N., Fournier, M., Audin, E., Kilchert, C., Grimes, J., and Vasiljeva, L. (2026) WDR82 Code Coordinates Transcription and pre-mRNA 3′-End Processing Through Regulated Phosphorylation. bioRxiv;DOI: https://doi.org/10.64898/2025.12. 17.694969 (accepted to Nature Communications)
Kus, K., Carrique, L., Fournier, M., Audin, E., Kilchert, C., Grimes, J., and Vasiljeva, L. (2025) The role of DSIF factor Spt5 in coordinating Pol II processivity and Xrn2 exoribonucleolysis for timely termination of transcription. Nature Communications 16(1):10. doi: 10.1038/s41467-024-55063-7.
Kus, K., Nielsen, S., Zenkin and Vasiljeva L. (2025) Conserved Pol II and RNA interacting protein is an anti-pausing transcription elongation factor. RNA DOI: 10.1261/rna.080765.125
Kus, K. and Vasiljeva, L. (2024) Exploring exoribonuclease activity using Fluorescence Anisotropy assay. Invited Chapter for Methods in Molecular Biology, Book by Springer.
Birot, A., Kus, K., Priest, E., Al Awash, A., Castello, A., Mohammed, S., Vasiljeva, L., and Kilchert, C. (2021) RNA-binding protein Mub1and the nuclear RNA exosome act to fine-tune environmental stress response. Life Sc. Alliance, 5, 2: e202101111.
Heo, D.H., Kuś, K., Grzechnik, P., Tan-Wong, S.M., Birot, A., Kecman, T., Nielsen, S., Zenkin, N. and Vasiljeva, L (2021) "Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription". Cell Reports, Sep 7; 36(10):109671 (PMID: 34496258; PMCID: PMC8441049)
Kilchert, C., Kecman, T., Priest, E., Hester, S., Aydin, E., Kuś, K., Rossbach, O., Castello, A., Mohammed, S. and Vasiljeva L (2020) "System-wide analyses of the fission yeast poly(A)+ RNA interactome reveal insights into organization and function of RNA-protein complexes". Genome Research, Jul; 30(7):1012-1026 (PMID: 32554781; PMCID: PMC7397868)
Kilchert, C., Hester, S., Castello, A., Mohammed, S. and Vasiljeva, L (2019) "Comparative poly(A)+ RNA interactome capture of RNA surveillance mutants". Methods in Molecular Biology, Springer, Edited by LaCava and Vanacova, eds. (HUMANA), vol. 2062, ch. 13;
ISBN 978-1-4939-9821-0.
Kecman, T., Kus, K., Heo, D. H., Birot, A., Mohamed, S. Geiss-Astggiante, L., Robinson C., and Vasiljeva, L. (2018) "Elongation to termination factors exchange mediated by PP1 phosphatase orchestrates RNA polymerase II transcription termination". Cell Reports, Oct2; 25(1):259-269. (PMID:30282034; PMCID: PMC6180485). https://www.ncbi.nlm.nih.gov/pubmed/30282034
The article was also highlighted in the University press release:
Watts, B.R, Wittmann, S., Kus, K., Wery, K., Gautier, C., Kilchert, K., Morillon, A., and
Vasiljeva, L. (2018) "Histone deacetylation promotes transcription silencing at facultative
heterochromatin". Nucleic Acids Res., 46(11):5426-5440.(PMID: 29618061; PMCID:PMC6009587).
Kecman and Vasiljeva, L. (2018) "Genome-wide approaches in fission yeast". Methods of Enzymology, 612:489-504. (PMID: 30502955).
Wittmann, S., Renner, M., Watts, B. R., Adams, O., Huseyin, M., Baejen, C., El Omari,
K., Kilchert, C., Heo, D. H., Kecman, T., Cramer, P., Grimes, J., and Vasiljeva, L. (2017)
"The conserved protein Seb1 drives transcription termination by binding RNA pol II and
nascent RNA". Nature Communications, 8:14861. (PMID:28367989; PMCID:PMC5382271).
https://www.ncbi.nlm.nih.gov/pubmed/28367989
The article was also highlighted in the news: https://www.bioch.ox.ac.uk/article/how-group-conserved-proteins-orchestrate-transcription-termination-eukaryotes
Kilchert, C., Wittmann, S., and Vasiljeva, L. (2016) "The nuclear RNA exosome complex: Regulation and Functions". Nature Reviews in Molecular Cell Biology, 17, 227-39.(PMID:26726035).
Kilchert, C., Wittmann, S., Passoni, M. Shah, S., Granneman, S., and Vasiljeva, L. (2015) "Regulation of mRNA Levels by Suicidal Introns that Induce Nuclear Decay". Cell Reports,13(11): 2504-2515. (PMID:26670050, PMCID: PMC4695336).
Shah, S., Kilchert, C., Wittmann, S., and Vasiljeva, L. (2014) "lncRNA recruits RNAi and the exosome to dynamically regulate pho1 expression in response to phosphate levels in fission yeast". Genes and Development 28, 231-244. (PMCID:PMC3923966).
https://www.ncbi.nlm.nih.gov/pubmed/24493644
Volanakis, A., Passoni, M., Hector, R., Kilchert, C., Granneman, S., and Vasiljeva, L. (2013) "Spliceosome Mediated-Decay (SMD) regulates expression of non-intronic genes in budding yeast". Genes and Development 27, 2025-38 (PMCID:PMC3792478)
https://www.ncbi.nlm.nih.gov/pubmed/24065768
Kilchert, C. and Vasiljeva, L. (2013) "mRNA quality control goes transcriptional". Biochemical Society Transactions Journal, 41, 1666-72.
https://www.ncbi.nlm.nih.gov/pubmed/24256272
Coy, S., Volanakis, A., Shah, S., and Vasiljeva, L. (2013) "The Sm complex is required for the processing of non-coding RNAs by the exosome". PLoS One 8(6), e65606.
(PMCID: PMC3675052)
https://www.ncbi.nlm.nih.gov/pubmed/23755256
Coy, S. and Vasiljeva, L. (2011) “RNA exosome” book chapter. "The exosome and heterochromatin: multilevel regulation of gene silencing". Landes Bioscience/Springer series: Advances in Experimental Medicine and Biology. ISBN:978-1-4419-7840-0.
https://www.ncbi.nlm.nih.gov/pubmed/21713681